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Posted: 14-Nov-23
Location: La Jolla, California
Salary: Open
Internal Number: 126117
This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 10/24/2023 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 11/02/2023. Eligible Special Selection clients should contact their Disability Counselor for assistance.
DESCRIPTION
The Moores Cancer Center is one of just 52 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 14 departments (12 clinical, 2 basic sciences), 3 professional schools (School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University, and the basic and translational research of the LaJolla Institute of Immunology. These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs.
As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center Business Office follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.
Perform tumor immunology studies consisting of murine models of cancer, animal breeding and genotyping, flow cytometry, single cell sequencing, immunohistochemistry, in vitro cell biology, and in vitro biochemistry. Murine tumor biology studies will include xenograft, orthotopic and spontaneous tumor growth and metastasis assays, immunological analysis, treatment of animals with experimental therapeutic agents by gavage, intravenous and intraperitoneal methods, breeding and genotyping of transgenic and mutant mice, and immune cell isolations. Perform major surgery, including small animal anesthesia. In addition, perform immunohistochemistry of frozen and fixed tissues or cells.
Conduct various in vitro cell biology techniques to include performing flow cytometry analysis of cell surface protein expression, cell adhesion and migration assays, transfecting cells with expression constructs, preparing lysates of cells in culture or tissues, quantification, ELISA assays, SDS PAGE, Western Immunoblotting and gel electrophoresis. Additionally, perform recombinant DNA techniques to include preparing plasmids and maintaining plasmid stocks, analyzing DNA and RNA purification, and conducting DNA electrophoresis, and RT-PCR.
Make innovative contributions regarding experimental design and methodology, using published scientific data in referred journals, as well as online using search engines and subject-specific websites, to make recommended changes to current protocols and practices. Troubleshoot problems for quality control on immunochemical and histochemical procedures. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Assume responsibility for lab maintenance and safety training. Provide training and work direction in areas of expertise as needed. Maintain and prepare accurate records of all experiments. Assist in the preparation of data for publication. Other duties assigned as needed.
MINIMUM QUALIFICATIONS
Advanced theoretical knowledge of molecular biology, biochemistry, physiology and cell biology or a Bachelor's of Science degree in an appropriate field of science or equivalent in education and experience.
Experience independently isolating bone marrow and blood cells for analysis by flow cytometry, immunoblotting, gene expression and cell adhesion assays.
Experience independently performing and directing in vivo experiments including the injection of growth factors, tumor cells and therapeutics, bleeding and tissue harvesting of mice and in performing minor surgery.
Experience independently performing and directing protein analysis techniques including protein determination, ELISA and Magpix assays and protein separation techniques.
Experience independently working with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in
Connections working at University of California San DiegoUC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.