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Posted: 25-Oct-23
Location: La Jolla, California
Salary: Open
Internal Number: 126031
UCSD Layoff from Career Appointment: Apply by 10/26/2023 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 11/07/2023. Eligible Special Selection clients should contact their Disability Counselor for assistance.
12/06/23 This position has changed levels from SRA 2 to SRA 3.
DESCRIPTION
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education, and patient care are intertwined and are integral to the goals of the department.
The Department has undergone significant growth in recent years and the expansion of the Department's research and clinical portfolio is expected to continue in the next few years.
Under general supervision, the incumbent will perform a variety of cellular and molecular assays and small animal experiments for different projects. The incumbent will process laboratory specimens for FACS, and use sorted cells to prepare bulk and single cell RNAseq, ATACseq and ChIPseq libraries. The incumbent will analyze data and interpret results independently and in collaboration with others. In addition, the incumbent will present, and prepare for publication results under the direction of the PI. The research assistant is expected to make contributions pertaining to experimental methodology by reviewing published scientific data, as well as online information using search engines and subject-specific websites, to make recommended changes to current protocols and practices.
Mouse work will include setting up timed breeding, setting up hyperoxia chamber experiments, mouse injection, dissection, and isolating immune cell populations. Performs other duties as assigned.
MINIMUM QUALIFICATIONS
Theoretical knowledge of cell biology, molecular biology, immunology or related field, as typically attained by an advanced degree, or an equivalent combination of education and experience.
Demonstrated experience of laboratory work with a good understanding of all the components of successful operation of a molecular biology lab.
Practical background with demonstrated ability in mouse colony management, breeding with timed pregnancies genotyping, harvesting lung, brain and retina and minor animal procedures, such as IP injection and gavage techniques.
Proven ability in computer skills, including Office and Excel.
Demonstrated ability to take direction, be detail oriented and follow step-wise instruction.
Expertise in molecular biology techniques including nucleic acid manipulation, electrophoresis, tissue and cell culture, and PCR required.
Ability to work independently and oversee others; demonstrated ability to work effectively as part of a team in a deadline-driven environment; good communication and analytical skills.
Ability to troubleshoot complex technical procedures.
Proven ability to work with a large number of samples with accuracy and efficiency.
Skills and knowledge in flow cytometry, especially fluorescence-activated cell sorting (FACS).
PREFERRED QUALIFICATIONS
- Experience in high-throughput platforms is preferred, such as experience with library preparation for Illumina Next Generation sequencing, including RNA-seq and ATAC-seq. In addition, experience with quantifying libraries with accuracy using quantitative PCR and Qubit fluorometer.
SPECIAL CONDITIONS
Job offer is contingent upon satisfactory clearance based on Background Check results.
Must be willing to vary work schedule to accommodate research needs.
Must be willing to work occasional evenings or weekends as required.
Must be willing and able to work with small animals and biohazardous materials, including human tissues.
Pay Transparency Act
Annual Full Pay Range: $64,506 - $103,727 (will be prorated if the appointment percentage is less than 100%)
Hourly Equivalent: $30.89 - $49.68
Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for