UCSD Layoff from Career Appointment: Apply by 9/14/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 9/23/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Perform standard laboratory procedures with Human Embryonic Stem Cells (hESC), neuronal precursor cells (NPCs), induced neurons (iNeurons), and mature neurons (mNeurons), and brain organoids. Under supervision, incumbent will work with PI and other lab members to refine protocols and to generate data from mutant cells for publication. Studies will include 1) General growth and maintenance of fibroblast and stem cell lines (adult and embryonic derived), 2) Gene manipulation of these stem cells with, CRISPR targeting, shRNA silencing or overexpression using transfection or lentivirus transduction, 3) Characterization of resultant phenotypes by molecular and imaging techniques, and 4) Molecular biology techniques to isolate RNA and quantify gene expression. Procedures will include molecular biology (generation of transgene constructs, PCR and RNA isolation) and microscopy as well as basic molecular and cellular biology techniques (Cell transfection, genomic screening, cloning, gene expression, immunocytochemistry, tissue culture and maintenance of biochemistry equipment). Independently perform tissue culture of primary cells, preparation of lentiviruses and viral infection techniques, maintain tissue culture facility stocks and inventory, perform regularly scheduled mycoplasma testing, ship and receive cell lines for collaborations. Perform cell culture electroporation in stem cells and independently perform routine biology and biology techniques including next generation sequencing techniques. Other tasks as required by the supervisor and project scientists. Incumbent will also be involved with general lab organization procedures.
Theoretical and/or practical knowledge in cellular and molecular biology, immunology, stem cell or related field as typically attained by a Bachelor's degree, or an equivalent combination of education and experience..
Demonstrated ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis, sterile technique.
Demonstrated experience in performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers, pH solutions, as well as various staining solutions.
Experience performing laboratory procedures with Human Embryonic Stem Cells (hESC) and, neuronal precursor cells (NPCs) and brain organoids.
Experience maintaining human embryonic stem cells, neuronal precursor cells, & hESC derived motor neurons in undifferentiated state.
Demonstrated experience generating lentiviral/retroviral culture medium for infection of hESCs, NPCs, & hESC derived motor neurons.
Experience working with DNA, RNA, protein in preparation for next generation sequencing or proteomics analysis.
Experience conducting experiments leading to differentiation patterns and the histological determination of specific phenotypes.
Experience with the operation of a personal computer and software such as Filemaker or other databases.
Proven ability to operate confocal, light, and fluorescence microscopes for documentation of results.
Demonstrated ability to follow and optimize complex and evolving step based cell culture protocols.
Demonstrated ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.
Experience with general lab maintenance including cleaning, stocking supplies.
Demonstrated ability to prioritize and perform efficiently.
Willing to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues.
Skill and ability to keep accurate data.
Demonstrated ability to read, comprehend, and discuss research materials.
Demonstrated ability to follow established Safety and Biohazard Material Guidelines.
Bachelor's degree preferred.
Willingness to work with animals, lentivirus, radioactive material, embryonic stem cells, human tissue and standard hazardous laboratory compounds.
Willing to work evenings and weekends to maintain workflow.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.