UCSD Layoff from Career Appointment: Apply by 08/31/2021 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 09/10/2021. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, the HIV Research Technician (SRA 2) will independently perform experiments involving a variety of standard molecular biology techniques, and/or a limited number of non-standard experimental procedures. Standard experiments include human immunodeficiency virus (HIV) viral load assays, DNA/RNA extraction and purification, cDNA synthesis, polymerase chain reactions, gel electrophoresis, and DNA sequencing. Non-standard experimental procedures include rapid autopsy and associated preparations, protocol adaptations, and various troubleshooting roles. Other duties also include independently processing human blood and clinical samples from HIV-infected donors. HIV Research Technician (SRA 2) will conduct and coordinate experiments and studies to assist post-docs, students, and visiting scientists within the lab. The technician will be independently responsible to handle other laboratory tasks including maintaining an accurate laboratory notebook, detailed inventory of clinical specimens, freezer inventory, ordering supplies, preparation of solutions, maintain a clean laboratory area, minor equipment maintenance, and other lab tasks as needed.
Theoretical knowledge of biology, molecular biology, or other related field typically acquired by a Bachelor's degree in a field related to the research being conducted, or equivalent years of experience.
Knowledge and demonstrated experience with standard molecular biology techniques including HIV viral load assays, DNA/RNA extraction and purification, cDNA synthesis, polymerase chain reactions, gel electrophoresis, and DNA sequencing.
Demonstrated knowledge of the operation of scientific and experimental laboratory equipment to include hand pipettors, pipetmans, pH meters, Mettler analytical and top-loading balances, plate washers, plate readers, agarose gel apparatus, thermocyclers, incubators, and various types of centrifuges.
Demonstrated organizational skills including the ability to organize and adapt the work schedule to accommodate experimental design.
Knowledge and demonstrated experience with sterile culture and aseptic techniques. This includes preparation of buffers and reagents.
Ability to keep organized, neat, and accurate laboratory records and notebooks; effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials.
Demonstrated computer proficiency, specifically software applications such as MS Word, and Excel.
Excellent organizational skills.
Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles. Ability to work with a variety of personnel from different laboratories.
Knowledge of biosafety techniques and practices.
Knowledge of proper manipulation of infectious specimens such as the use of Biological Hazard Level III and III bio-containment facilities and cabinets, decontamination of work areas and proper laboratory attire.
Knowledge of high throughput specimen processing, sequencing, and sequence data management.
Demonstrated knowledge and experience of processing blood, CSF, cultured cells and their supernatants infected with HIV and other human retroviruses.
Employment is subject to a criminal background check.
Must be willing to process culture cells and human specimens infected with HIV and other human retroviruses.
Must be willing to work with hazardous chemicals.
Must be able to able to work flexible hours to meet deadlines.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.