UCSD Layoff from Career Appointment: Apply by 04/29/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 05/10/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, perform at full operational skill level, conduct experiments in cell signaling and characterize interactions involving growth factors and other proteins in the fields of cancer and neuronal biology. Independently perform experiments making technical determinations and/or observations related to research projects. Independently perform PCR, cell-based assays, cell culture, protein blotting, and flow cytometry. Conduct limited variety of non-standard procedures. Conduct animal experiments with both mice and rats including histology, staining, and immunohistochemistry. Data processing and presentation and statistical analysis. Contribute to lab administration, compliance, and maintenance.
Bachelor's degree in biomedical or a related science with two (2+) or more years of recent, directly related work experience.
Experience and ability to initiate and maintain multiple different eukaryotic cell lines including freezing, thawing, storage, and plating cells in preparation for experiments. Experience with sterile techniques, trouble shooting of contamination problems, and care of incubators and liquid nitrogen freezers.
Demonstrated experience with immunohistochemistry, histology processing, embedding, sectioning, and staining.
Theoretical knowledge of molecular biology techniques and genetics and experience in extracting DNA/RNA, PCR, PT-PCR, qPCR.
Proven experience in protein chemistry/purification, gel electrophoresis and transfers, various gel and membrane stains, western blots, and densitometry and analysis on resulting data.
Demonstrated experience with mouse and rat handling, surgeries, anesthesia, injections, euthanasia, and tissue harvest.
Knowledge of bio, chemical, and radiation safety techniques and practices. Ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Excellent communication and interpersonal skills over a wide range of situations and individuals needed to interact professionally and effectively in the work environment in person, on the phone, and in writing to a diverse population.
Knowledge of proper formats, grammar, and spelling in English and proofreading ability sufficient to compose, edit, and publish professional correspondence as well as effectively and accurately document research procedures. Ability to read, comprehend, discuss, and present research materials.
Experience making independent decisions, analyzing situations, problem solving, and initiating action for laboratory maintenance. Ability to work independently. Ability to follow written and verbal protocols, techniques, and instructions.
Strong organizational skills to order and maintain lab reagents and supplies, and keep accurate lab records and computer files. Experience in ordering, maintaining, trouble-shooting, and calibrating general laboratory equipment (e.g. centrifuges, incubators, microscopes, water baths, etc.).
Proficient experience with the operation of a personal computer and software applications, such as Microsoft Office (Word, Excel, Access), Photoshop, Acrobat.
Experience with gel/blot imaging and microscopic imaging techniques for both fluorescence and bright field applications and with digital histology image software assisted image processing and analysis.
PhD degree and postdoc training in liver research.
Six (6) years of experience in liver research, including liver biology, architecture, hepatic cell lineage tracing, regeneration, fibrosis and cancer.
Experience with UCSD ordering, EH&S, and facilities policies and business systems.
Employment is subject to a criminal background check.
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