The qualifications of this posting have been updated as of 3/29/2021.

DESCRIPTION

Our laboratory studies cardiovascular disease using human blood samples and transgenic mouse models.

This position requires the ability to independently performing basic molecular biology techniques, managing a mouse colony consisting of multiple transgenic lines, and general lab maintenance. Salary and position title will be commensurate with proficiency with the following skills/experience:Independently perform basic routine molecular biology techniques such as ELISA, DNA/RNA extraction, PCR, qPCR, sample preparation for NGS sequencing, SDS PAGE, Western Blot, flow cytometry, lipoprotein purification, and various multi-well plate based assays.Organize and maintain a large mouse colony comprised of multiple transgenic mouse lines. Perform genotyping with PCR and maintain detailed mouse colony records. Manage breeding programs with the most efficient strategies. Independently perform procedures involving mice including phlebotomy, induction and maintenance of rodent anesthesia, minor surgeries that include telemetry ECG/BP sensor- transmitter implantation, pre- and post- operational animal cares in BSL2 facilities. Provide care for mice with dermatitis including nail trimming and topical antibiotic treatments. Perform mouse euthanasia, perfusion, dissection, tissue/sample collection and processing, tissue histology including sectioning, staining, and microscopy, data collection and analysis.

Lab management including ordering and receiving supplies, prepare biohazards waste for collection according to safety regulations, maintaining a functional clean and orderly lab, maintain equipment, train and orient all new personnel involved with animal use, biohazard and safety training, controlled substances training, organize and retain all records of ordering, training, animal records, protocols, etc.

Must be familiar with IACUC and AAALAC requirements.

Depending on the experience of the selected candidate, this position will be hired at the Staff Research Associate I or Staff Research Associate II level.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of cardiovascular physiology and pharmacology.

• Proven experience in a cardiovascular research laboratory; including experience with animal health and mouse genetics.

• Proven experience in working with multi-well plate based assays (i.e. ELISA).

• Demonstrated experience handling mice, managing transgenic mouse colonies and maintaining animal records according to UCSD Animal Care Program protocol.

• Demonstrated ability to analyze and develop statistical data.

• Working knowledge of laboratory practices, protocols and safety requirements.

• Demonstrated problem-solving and critical thinking skills.

• Ability to maintain effective working relationships with other staff members, researchers, faculty and students.

• Familiarity with University/NIH/AAALAC policies and compliance procedures.

• Proven ability to learn techniques quickly and to make adjustments as necessary.

PREFERRED QUALIFICATIONS

• Experience with UCSD Animal Subject procedures.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.
• Must be willing to work with small animals including mice.