DESCRIPTION

The Coufal lab studies neuroinflammation and the role of microglia, the inflammatory cell type of the brain, in neurodevelopment, aging, and both idiopathic and genetic neurologic disease using a variety of in vitro and in vivo models and stem cell derived microglia. The position is as the lab manager, with preference given to individuals with lab management experience with a goal of participating not only in science, but also regulatory, financial, and compliance areas.

Under supervision of the Principal Investigator and in conjunction with postdoctoral researchers in the Principal Investigator's laboratory, the Staff Research Associate I incumbent will participate in a variety of different techniques including induced pluripotent stem cell research, differentiation of induced pluripotent stem cells to a variety of cell fates including microglia, neurons, astrocytes, and cerebral organoids, and will learn and utilize general molecular biology experiments including: preparing RNA from cells and tissue, cDNA synthesis and quantitative PCR, performing RNAseq and ChIPseq library preparations, cloning and general molecular biology techniques, western blotting, immunohistochemistry of cells and organoids, and cryostat sectioning. There is the opportunities to learn and master a variety of techniques and to contribute not only technically but also to experimental design as able.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of molecular biology or cellular biology generally acquired through a Bachelor's degree or an equivalent combination of education and experience.

• Experience in and knowledge of general molecular biology techniques, and use of common laboratory equipment.

• Experience performing RNA purification, cDNA synthesis, and quantitative PCR assays.

• Demonstrated ability to communicate effectively in standard English, in writing, electronically, and by spoken word with lab members and vendors.

• Ability to assist lab personnel in tissue culture, including stem cell culture

• Able to work independently and as part of a team, establishing and maintaining good workingrelationships with P.I., supervisor, postdoctoral scholars, and other members of the research group.

• Able to take direction, be detail oriented, follow step-wise protocols, and perform repetitive tasks in a fast-paced laboratory environment. Able to learn new procedures.

• Experience with and knowledge of laboratory safety including radioactive/biohazard handling & disposal techniques, & the ability to ensure laboratory compliance with required safety regulations.

• Experience with all aspects of mouse colony management including mouse genotyping, husbandry methods, breeding, weaning, etc.

• Experience with animal experiments, animal protocol requirements, and training in animal treatment.

• Knowledge of basic lab operations, procedures, and proper use and maintenance of lab equipment.

PREFERRED QUALIFICATIONS

• Bachelor's Degree or higher.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.
• Must be willing to vary work schedule to accommodate research needs, including occasional overtime, nights, weekends.
• Must be able to work in environment where biological and chemical hazards are present.
• Must be able to work with human cells including established induced pluripotent stem cells and human embryonic stem cells.
• Must be willing to work with mice.