DESCRIPTION

The UCSD Department of Pharmacology is one of two basic science units in the School of Medicine where world-class researchers investigate the molecular basis of human diseases. The faculty are internationally renowned for their strength in elucidating basic cellular and molecular mechanisms of signal transduction that lead to innovative drug target identification and ultimately to drug discovery. The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines, and provide diversity to meet the interests of a broad spectrum of students and scholars. More than one hundred trainees at the graduate student and postdoctoral level, as well as a staff of more than 50 research technicians and department administrators interact closely with the faculty. The diverse mix of ages, backgrounds, and talents creates a robust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced and interested individuals to become a part of our dynamic exploratory research and educational environment.

In collaboration with senior scientists, under close supervision, the incumbent will be responsible of the evaluation of innate inflammatory response in acute respiratory syndrome mouse models in the context of obesity. Will be responsible for monitoring mouse body weight during the course of the experiments, perform treatment (intraperitoneal and intratracheal administration), and providing special diets weekly. Perform primary mouse macrophage culture and mouse cell line culture and maintenance. Execute histological analysis of the samples by immunohistochemistry. Will assist with In vitro studies that will include several biochemistry and molecular and cellular biology techniques, including ELISA, DNA and mRNA isolation, QPCR, protein isolation and WB, Immunofluorescence. Interact with faculty, postdoctoral scholars, students and research technician in a large laboratory.

MINIMUM QUALIFICATIONS

• Theoretical knowledge of chemistry, biochemistry or biology and laboratory experience.

• Experience working with mice, preferred knowledge of lung pathology or expertise with obesity-related mouse models.

• Demonstrated experience with basic laboratory procedures and equipment, including sterile techniques, preparation of buffers and media, cell culture, PCR, protein analysis and purification.

• Knowledge of/or experience maintaining and working with primary cells or cell lines on culture.

• Knowledge and understanding of laboratory safety and ability to work carefully with potentially harmful agents.

SPECIAL CONDITIONS

• Employment is subject to a criminal background check.

• Occasional evenings and weekends may be required.

• Must be willing to work with hazardous materials.

• Must be willing to work with rodents.