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Staff Research Associate III
University of California, San Diego
The effects of the COVID-19 pandemic have impacted UC San Diego Campus hiring. UCSD Campus will continue to recruit for essential positions and we ask for your understanding and patience with our application process during this unprecedented time. For more information regarding steps we are taking to keep applicants and staff safe during this time, please click here. UCSD Layoff from Career Appointment: Apply by 12/09/20 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Special Selection Applicants: Apply by 12/18/20. Eligible Special Selection clients should contact their Disability Counselor for assistance. Job posting will remain open until position is filled. DESCRIPTIONUnder general supervision, the incumbent will be responsible for undertaking research projects in collaboration with the Principal Investigator (PI) making independent contributions to research and experiments regarding tissue culture of mammalian cell lines. The incumbent will work independently to make determinations and design experiments in yeast work, which will include yeast strain genetic manipulation, yeast transformation of DNA and selection on the appropriate yeast cell plates, preparing yeast cell extracts for various biochemical analyses, immunofluorescence, live cell imaging and RNA analyses. In addition, the incumbent will grow established mammalian cell lines and primary mouse cells to generate protein extracts or nucleic acid (both DNA and RNA) or immunofluorescence or live-cell imaging samples. Furthermore, the incumbent will generate frozen mammalian cell lines and yeast strain stocks. Mammalian cell work will also include standard tissue culture cell maintenance, transfection of plasmid DNA, and selection and cloning of the appropriate transformants on the selectable media. Other duties include: read and abstract articles of value in the prosecution of broad research problems; analyze and interpret genetic screens. Incumbent will participate in writing and publishing manuscripts in international journals, and attend and present research results at laboratory meetings. Finally, incumbent will provide work direction to laboratory staff, undergraduate and graduate students in overseeing lab databases of strains, cell lines and reagents. Perform other duties as assigned. QUALIFICATIONS
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