Provide support to research projects on reproductive physiology of ruminant species, primarily beef cattle, but potentially including dairy cattle, buffaloes, and small ruminants. Typical duties include: • Plan and participate in research projects. • Performance of various biochemical and molecular biological analyses using a variety of equipment dependent on the research objectives and experimental design established in conjunction with the faculty member, i.e, ELISA, DNA extraction, PCR (end-point and real-time), Wester blotting, Immunomicroscopy, Immunohistochemistry, and Immunofluorescence and sperm count, etc. • Standardization of methods and develop new procedures applicable to the experimental and research protocols of laboratory users. • Assist in grant proposal preparation, literature searches, retrieval of material, data collection, processing, basic statistical evaluation, and assistance with preparation of manuscripts. • Assist in working with computer applications, i.e., basic word processing, graphics, slide preparation, statistical programs and data management programs, including gene expression, omics and “big-data”. • Collaborate in data and sample collection and other field activities, including but not limited to: rectal palpation and ultrasonography, collection of biological material (i.e.: blood, biopsies, cells, etc.), feeding animals, among others. • Coordinates special studies or projects as directed. Laboratory Management: • Maintenance of laboratory equipment and laboratory physical area, including ordering supplies, equipment and inventory record keeping. • Coordinate with research staff on issues which impact laboratory services/research. • Maintain liaison with investigators, research personnel and staff to solve specific operating problems and improve technical activities. • Acts as an advisor to meet schedules and/or resolve technical problems. • Administer and coordinate the technical activities, educational programs, and infrastructure related to the operation of laboratory. • Perform duties as required or deemed appropriate to the accomplishment of the responsibilities and functions of the laboratory area. • Recommends, drafts and implements operating policies and procedures. • Assist in the interpretation and implementation of laboratory policies and procedures. • Insure that Standard Operating Procedures are developed, maintained and applied. • Coordinate and maintain EHS standards of oversight and safety for all laboratory area, specialized scientific or hazardous materials stores. • Ensure compliance with federal regulations for hazardous waste disposal. • Maintain inventory and maintenance of sensitive scientific materials/instruments. • Research and evaluate new products and equipment, compare and negotiate vendor prices. • Prepare and monitor operating budget. Recommend purchases. • Use purchasing card and purchase orders as per policies and regulations. • Directs the maintenance program for laboratory equipment. • Coordinates scientific services with faculty using the lab. Interact with laboratory personnel, faculty, staff and students in a spirit of cooperation.
• Knowledge of principles of chemistry, immunology, biochemistry, molecular biology and cell culture. • Ability to follow standard operating procedures and properly document procedures • Ability to perform ELISA, DNA extraction, PCR (end-point and real-time), Wester blotting, Immunomicroscopy, Immunohistochemistry, and Immunofluorescence and sperm count, etc. • Manual dexterity and expertise in the setup operation and maintenance of equipment. • Skill in use of standard and specialized laboratory equipment and basic laboratory procedures. • Skills in the use of various computerized laboratory equipment. • Ability to maintain accurate lab records and to handle data efficiently • Laboratory maintenance: maintain lab in good order, maintain supplies and equipment, review and update safety and compliance requirements • Develop research design and methods and conduct these experiments in the laboratory • Write reports and scientific papers that describe and interpret research findings • Proficient with Microsoft Office-Excel, Word, PowerPoint. • Work cooperatively with other research labs at the University of Florida and elsewhere • Ability to effectively work with co-workers, to plan, organize and coordinate work assignments. • Ability to communicate with co-workers, professional staff, residents and clinicians. • Strong organizational and time management skills. • Independent acquisition of advanced technical skills and computer applications. • Experience with large animals, including sample collection and anatomical knowledge.
Internal Number: 514315
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